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cellstain hoechst 33342 solution  (Dojindo Labs)


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    Structured Review

    Dojindo Labs cellstain hoechst 33342 solution
    Cellstain Hoechst 33342 Solution, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hoechst+33342/Cellstain+Hoechst+33342+solution/custom%40h342%4041794052
    Average 96 stars, based on 388 article reviews
    cellstain hoechst 33342 solution - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Tri‐Culture System Reveals an Activation Cascade From Microglia Through Astrocytes to Neurons During Neuroinflammation
    Article Snippet: Subsequently, blocking and permeabilization were performed using PBS (−) supplemented with 1% BSA and 0.2% Triton X‐100 for 1 h. Then, the cells were incubated overnight at 4°C with primary antibody solution (anti‐IBA1, rabbit, 1:800, Fujifilm Wako, cat. no. 019‐19741; anti‐MAP2, chicken, 1:800, Novus Biologicals, cat. no. NB300‐213; anti‐GFAP, mouse, 1:3000, Merck Millipore, cat. no. MAB3402; anti‐IBA1, goat, 1:500, Fujifilm Wako, cat. no. 011‐27991; anti‐NF‐κB, rabbit, 1:400, Cell Signaling, cat. no. 8242; anti‐NeuN, mouse, 1:700, Sigma Aldrich, cat. no. MAB377). .. After three times of PBS (−) wash, cells were incubated with secondary antibodies and Hoechst 33342 (Dojindo, cat. no. 346‐07951) for 1 h at room temperature (anti‐rabbit Alexa Fluor 488, goat, 1:1000, Thermo Fisher Scientific, cat. no. A11008; anti‐chicken Alexa Fluor 594, goat, 1:1000, Thermo Fisher Scientific, cat. no. A11042; anti‐mouse Alexa Fluor 647, goat, 1:1000, Thermo Fisher Scientific, cat. no. A32728; anti‐goat Alexa Fluor 488, donkey, 1:1000, Thermo Fisher Scientific, cat. no. A11055; anti‐rabbit Alexa Fluor 594, donkey, 1:1000, Thermo Fisher Scientific, cat. no. A32754). .. After three times wash with PBS (−), images were captured using ECLIPSE Ti (Nikon) and CellVoyager CQ1 (Yokogawa Electric Corporation).

    Article Title: Tri-Culture System Reveals an Activation Cascade From Microglia Through Astrocytes to Neurons During Neuroinflammation.
    Article Snippet: Subsequently, blocking and permeabilization were performed using PBS (−) supplemented with 1% BSA and 0.2% Triton X- 100 for 1 h. Then, the cells were incubated overnight at 4°C with primary antibody solution (anti- IBA1, rabbit, 1:800, Fujifilm Wako, cat. no. 019- 19741; anti- MAP2, chicken, 1:800, Novus Biologicals, cat. no. NB300- 213; anti- GFAP, mouse, 1:3000, Merck Millipore, cat. no. MAB3402; anti- IBA1, goat, 1:500, Fujifilm Wako, cat. no. 011- 27991; anti- NF- κB, rabbit, 1:400, Cell Signaling, cat. no. 8242; anti- NeuN, mouse, 1:700, Sigma Aldrich, cat. no. MAB377). .. After three times of PBS (−) wash, cells were incubated with secondary antibodies and Hoechst 33342 (Dojindo, cat. no. 346- 07951) for 1 h at room temperature (anti- rabbit Alexa Fluor 488, goat, 1:1000, Thermo Fisher Scientific, cat. no. A11008; anti- chicken Alexa Fluor 594, goat, 1:1000, Thermo Fisher Scientific, cat. no. A11042; anti- mouse Alexa Fluor 647, goat, 1:1000, Thermo Fisher Scientific, cat. no. A32728; anti- goat Alexa Fluor 488, donkey, 1:1000, Thermo Fisher Scientific, cat. no. A11055; anti- rabbit Alexa Fluor 594, donkey, 1:1000, Thermo Fisher Scientific, cat. no. A32754). ..

    Staining:

    Article Title: Maternal vitamin A deficiency suppresses browning of white adipose tissue in offspring.
    Article Snippet: doi:10.1111/febs.70523 Beige adipocytes transiently emerge in white adipose tissue (WAT) during early postnatal development in mice (postnatal browning) independent of sympathetic innervation.. However, the underlying mechanism remains unclear.. Retinoic acid (RA), an active metabolite of vitamin A, has been reported to promote angiogenesis and browning.

    Concentration Assay:

    Article Title: Fusion protein of pentamer and gB of cytomegalovirus, and vaccine containing said fusion protein
    Article Snippet: This was washed with 0.5% BSA/PBS solution, 100 μL/well of stain solution in which Goat Anti-Mouse IgG H & L (Alexa Fluor488) (Abcam plc. .. : abl50113) was diluted to a final concentration of 1000 times, and Hoechst 33342 (DOJINDO LABORATORIES: 346-07951) was diluted to a final concentration of 500 times with 0.5% BSA/PBS solution was added, and the mixture was left to stand at room temperature for 1 hour. ..

    Microscopy:

    Article Title: In vitro polarized macrophages ameliorate adverse cardiac remodeling in a mouse model of transverse aortic constriction
    Article Snippet: .. Actin filaments and nuclei were counterstained with AF488 phalloidin (1:400, Thermo Fisher Scientific, A12379) and Hoechst 33342 (1:100, Dojindo, Kumamoto, H342-10), respectively and then visualized under a BZ-X810 microscope (Keyence, Osaka, Japan). ..



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